KMID : 1134820200490030236
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Journal of the Korean Society of Food Science and Nutrition 2020 Volume.49 No. 3 p.236 ~ p.243
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A Novel Strain of Saccharomyces servazzii, Ceb-kc-011 from Young Radish Kimchi: Optimization of the Growth Condition and Inhibition of ¥á-Glucosidase by Bioconverted Rhynchosia nulubilis Using Ceb-kc-011
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Park Chan-Ho
Choi Yae-Rim Yum Kyu-Jin Kim Min-Jeong
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Abstract
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Food bioconversion and fermentation is a process wherein microorganisms convert food materials to degradation products, including organic acids and bioactive substances. Rhynchosia nulubilis, which is used traditionally as a food and herbal medicine in Asia, has also been biotransformed by microorganisms, and fermented R. nulubilis has anti-hyperglycemic, anti-hyperlipidemic, and anti-obesity activity. On the other hand, the microbes involved in the fermentation of R. nulubilis remain unknown. In particular, the role of yeast in inducing the biotransformation of R. nulubilis has yet to be examined. This study assessed the adaptation of yeast isolated from young radish (Yeolmoo) kimchi for inducing bioconversion using R. nulubilis. A novel yeast strain was isolated from young radish kimchi and called Saccharomyces servazzii Ceb-kc-011 by 18S rRNA gene sequence analysis. The results showed that the optimal culture conditions for Ceb-kc-011 were YPD media [yeast 1% (w/v), peptone 2% (w/v), D-glucose 2% (w/v)], 25¡ÆC, and a 24-hour incubation time under aerobic conditions. When R. nulubilis was added to the media, the growth of S. servazzii Ceb-kc-011 was indicated by the presence of yeast and peptones. Still, bioactive substances were produced in the absence of yeast and peptones (2% D-glucose and 4% R. nulubilis). Bioconverted R. nulubilis inhibited ¥á- glucosidase activity (41.43¡¾0.25%). In conclusion, the bioconversion of R. nulubilis using a specific microorganism was observed, highlighting its potential for the production of anti-diabetic agents through bioconversion.
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KEYWORD
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Saccharomyces servazzii, Ceb-kc-011, bioconversion of Rhynchosia nulubilis, fermented Rhynchosia nulubilis, ¥á-glucosidase inhibition
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